Two lions at the Etawah Safari Park in the northern Indian state Uttar Pradesh have tested positive for COVID-19, according to officials at the park.
The samples were sent for testing after both the lions were found to have high temperatures.
Authorities added that the pair were in stable condition. They have been kept in self-isolation with their health being monitored regularly.
This comes after eight lions had tested positive for the COVID-19 at the Nehru Zoological Park in Hyderabad earlier this week.
The Ministry of Environment, Forest, and Climate Change had previously stated that based on experience with other zoo animals who had also tested positive for COVID-19 across the globe, there is "no factual evidence that animals can transmit the disease to humans any further."
In a strong criticism of the Uttar Pradesh government's handling of the crisis, the state's top court said Tuesday that deaths of COVID-19 patients due to lack of oxygen is a "criminal act and not less than a genocide," according to a judgment posted on the court's website.
India's second wave has hit its major cities and populous states hard. Cases in the capital, New Delhi, began climbing in late March, accelerating rapidly in April—and as the virus spread from state to state, other parts of the country began to see their own surge in cases, sometimes weeks after the central hotspots.
The world's second most populous country reported its highest number of coronavirus deaths Saturday with 4,187 recorded over a 24-hour period, according to figures released by the country's health ministry. This is the first time the daily number of deaths has exceeded 4,000, bringing the total figure to 238,270.
India also added 401,078 new COVID-19 cases, the third day in a row that over 400,000 cases have been recorded. The total number of cases stands at 21,892,676.